Effect of glutathione on radioactivity and antibacterial activity of 14C-bleomycin in organs.

I performed an experiment on the relationship between 14C-Bleomycin and Glutathione in 'vivo; especially on the relationship of radioactivity and antibacterial activity, and the results will be reported here. 1) When Bleomycin and Glutathione were administered simultaneously and animals were killed after 30 minutes, decrease in Bleomycin concentration in every organ was observed. The decrease rate in tumor was especially great (radioactivity: 80.3%, antibacterial activity: 78.4%). 2) When Glutathione is administered 15 minutes after administration of Bleomycin and the animals were sacrificed after 30 minutes, the decrease rate was highest in the skin followed by that in tumor and extremeiy low in the lung. 3) When Glutathion was given 30 minutes after Bleomycin administration and the animals sacrificed after 60 minutes, the decrease rate of intraorgan Bleomycin concentration was in the order of lung> tumor> skin.